ABSTRACT
Many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). In a previous study, we isolated and characterized Type I cystatin (CsStefin-1) of the liver fluke, Clonorchis sinensis. To investigate whether the CsStefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (DSS) and treated them with recombinant CsStefin-1 (rCsStefin-1). The disease activity index (DAI) increased in DSS only-treated mice. In contrast, the DAI value was significantly reduced in rCsStefin-1-treated mice than DSS only-treated mice. In addition, the colon length of DSS only-treated mice was shorter than that of rCsStefin-1 treated mice. The secretion levels of IFN-gamma and TNF-alpha in the spleen and mesenteric lymph nodes (MLNs) were significantly increased by DSS treatment, but the level of TNF-alpha in MLNs was significantly decreased by rCsStefin-1 treatment. IL-10 production in both spleen and MLNs was significantly increased, and IL-10+F4/80+ macrophage cells were significantly increased in the spleen and MLNs of rCsStefin-1 treated mice after DSS treatment. In conclusion, rCsStefin-1 could reduce the intestinal inflammation occurring after DSS treatment, these effects might be related with recruitment of IL-10 secreting macrophages.
Subject(s)
Animals , Female , Mice , Antigens, Differentiation/analysis , Clonorchis sinensis/enzymology , Colon/pathology , Cystatins/metabolism , Cytokines/metabolism , Dextran Sulfate/toxicity , Helminth Proteins/metabolism , Immunologic Factors/metabolism , Inflammation/chemically induced , Interleukin-10/analysis , Intestines/drug effects , Lymph Nodes/immunology , Macrophages/chemistry , Mice, Inbred C57BL , Severity of Illness Index , Spleen/immunologyABSTRACT
En respuesta a la cirugía se producen cambios vasculares periféricos que aseguran una composición corporal, concentración electrolítica, volumen vascular, tonicidad y metabolismo ácido-base adecuados tanto para el trauma quirúrgico como para las características de salud o enfermedad del paciente. Estos cambios están modulados por factores neuroendocrinos hipotálamo-hipofisiarios, renales, suprarrenales y cardiacos
Subject(s)
Amino Acids, Peptides, and Proteins , Homeostasis/physiology , Immunologic Factors/metabolism , Neurosecretory Systems/physiology , Proteins/biosynthesisABSTRACT
En la respuesta inflamatoria local a la cirugía intervienen diversas substancias tales como histamina, kalikreínas, prostaglandinas, leucotrienos, complemento, interleucinas, factor de necrosis tumoral, factor derivado de plaquetas y radicales de oxígeno, que modulan la formación de edema. Migración de leucocitos y reparación tisular necesarios para existencia de condiciones microambientales satisfactorias para la nutrición, crecimiento y regeneración tisulares